› 3w9j

X-ray diffraction
3.15Å resolution

Structural basis for the inhibition of bacterial multidrug exporters

Released:
DOI: 10.2210/pdb3w9j/pdb
PDB entry 3w9j coloured by chain, front view.
PDB entry 3w9j coloured by chain, side view.
PDB entry 3w9j coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural basis for the inhibition of bacterial multidrug exporters.
Nakashima R, Sakurai K, Yamasaki S, Hayashi K, Nagata C, Hoshino K, Onodera Y, Nishino K, Yamaguchi A
Nature 500 102-6 (2013)
PMID: 23812586

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance protein MexB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 1052 amino acids
Theoretical weight: 113.71 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P52002 (Residues: 1-1046; Coverage: 100%)
Gene names: PA0426, mexB
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand LMT 15 x LMT
Ligand P9D 2 x P9D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P1
Unit cell:
a: 126.204Å b: 137.034Å c: 152.275Å
α: 85.75° β: 68.93° γ: 87.39°
R-values:
R R work R free
0.206 0.202 0.279
Expression system: Escherichia coli

Quick links

Citations

43 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
42 more

15 mentions without citation

Structural and Functional Diversity of Resistance-Nodulation-Cell Division Transporters.
Klenotic et al. (2021)
14 more