› 3wa1

X-ray diffraction
1.75Å resolution

Crystal structure of BinB: A receptor binding component of the binary toxin from Lysinibacillus sphaericus

Released:
DOI: 10.2210/pdb3wa1/pdb
PDB entry 3wa1 coloured by chain, front view.
PDB entry 3wa1 coloured by chain, side view.
PDB entry 3wa1 coloured by chain, top view.
Source organism: Lysinibacillus sphaericus
Primary publication:
Crystal structure of BinB: a receptor binding component of the binary toxin from Lysinibacillus sphaericus.
Srisucharitpanit K, Yao M, Promdonkoy B, Chimnaronk S, Tanaka I, Boonserm P
Proteins 82 2703-12 (2014)
PMID: 24975613

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Binary larvicide subunit BinB Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 44.8 KDa
Source organism: Lysinibacillus sphaericus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18568 (Residues: 19-407; Coverage: 87%)
Gene names: binB, sph04
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6222
Unit cell:
a: 95.21Å b: 95.21Å c: 154.891Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.21
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Bacillus thuringiensis toxins: an overview of their biocidal activity.
Palma et al. (2014)
5 more

3 mentions without citation

De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure.
Colletier et al. (2016)
2 more