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Structure of novel enzyme in mannan biodegradation process 4-O-β-D-mannosyl-D-glucose phosphorylase MGP.

Nakae S, Ito S, Higa M, Senoura T, Wasaki J, Hijikata A, Shionyu M, Ito S, Shirai T
J Mol Biol 425 4468-78 (2013)
Related entries: 3was, 3wat, 4kmi

Cited: 22 times
EuropePMC logo PMID: 23954514

Abstract

The crystal structure of a novel component of the mannan biodegradation system, 4-O-β-D-mannosyl-D-glucose phosphorylase (MGP), was determined to a 1.68-Å resolution. The structure of the enzyme revealed a unique homohexameric structure, which was formed by using two helices attached to the N-terminus and C-terminus as a tab for sticking between subunits. The structures of MGP complexes with genuine substrates, 4-O-β-D-mannosyl-D-glucose and phosphate, and the product D-mannose-1-phosphate were also determined. The complex structures revealed that the invariant residue Asp131, which is supposed to be the general acid/base, did not exist close to the glycosidic Glc-O4 atom, which should be protonated in the catalytic reaction. Also, no solvent molecule that might mediate a proton transfer from Asp131 was observed in the substrate complex structure, suggesting that the catalytic mechanism of MGP is different from those of known disaccharide phosphorylases.

Articles - 3wau mentioned but not cited (1)

  1. Structural bases for N-glycan processing by mannoside phosphorylase. Ladevèze S, Cioci G, Roblin P, Mourey L, Tranier S, Potocki-Véronèse G. Acta Crystallogr D Biol Crystallogr 71 1335-1346 (2015)


Reviews citing this publication (6)

  1. Enzymatic synthesis using glycoside phosphorylases. O'Neill EC, Field RA. Carbohydr Res 403 23-37 (2015)
  2. Glycoside phosphorylases: structure, catalytic properties and biotechnological potential. Puchart V. Biotechnol Adv 33 261-276 (2015)
  3. Diversity of phosphorylases in glycoside hydrolase families. Kitaoka M. Appl Microbiol Biotechnol 99 8377-8390 (2015)
  4. Functions, structures, and applications of cellobiose 2-epimerase and glycoside hydrolase family 130 mannoside phosphorylases. Saburi W. Biosci Biotechnol Biochem 80 1294-1305 (2016)
  5. Discovery and Biotechnological Exploitation of Glycoside-Phosphorylases. Li A, Benkoulouche M, Ladeveze S, Durand J, Cioci G, Laville E, Potocki-Veronese G. Int J Mol Sci 23 3043 (2022)
  6. The use of neutron scattering to determine the functional structure of glycoside hydrolase. Nakamura A, Ishida T, Samejima M, Igarashi K. Curr Opin Struct Biol 40 54-61 (2016)

Articles citing this publication (15)



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