› 3wgi

X-ray diffraction
3.25Å resolution

Crystal structure of RSP in complex with beta-NAD+ and operator DNA

Released:
DOI: 10.2210/pdb3wgi/pdb
PDB entry 3wgi coloured by chain, front view.
PDB entry 3wgi coloured by chain, side view.
PDB entry 3wgi coloured by chain, top view.
Source organism: Thermoanaerobacter ethanolicus JW 200
Primary publication:
Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes.
Zheng Y, Ko TP, Sun H, Huang CH, Pei J, Qiu R, Wang AH, Wiegel J, Shao W, Guo RT
J Struct Biol 188 195-204 (2014)
PMID: 25463021

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112706 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Redox-sensing transcriptional repressor Rex Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 25.47 KDa
Source organism: Thermoanaerobacter ethanolicus JW 200
Expression system: Escherichia coli
UniProt:
  • Canonical: D5KM69 (Residues: 1-224; Coverage: 100%)
Gene name: rex
Sequence domains:
Structure domains:
DNA (5'-D(*TP*AP*GP*AP*TP*TP*GP*TP*TP*AP*AP*TP*CP*GP*AP*TP*TP*AP*AP*CP*AP*AP*TP*C)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: Thermoanaerobacter ethanolicus JW 200
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NAJ 4 x NAJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 95.99Å b: 116.439Å c: 132.887Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.228
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Redox-Based Transcriptional Regulation in Prokaryotes: Revisiting Model Mechanisms.
Sevilla et al. (2019)
6 other articles

3 mentions without citation

Structural insights into the BRAF monomer-to-dimer transition mediated by RAS binding.
Martinez Fiesco et al. (2022)
2 more