› 3wi3

X-ray diffraction
2.4Å resolution

Crystal Structure of the Sld3/Treslin domain from yeast Sld3

Released:
DOI: 10.2210/pdb3wi3/pdb
PDB entry 3wi3 coloured by chain, front view.
PDB entry 3wi3 coloured by chain, side view.
PDB entry 3wi3 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin.
Itou H, Muramatsu S, Shirakihara Y, Araki H
Structure 22 1341-1347 (2014)
PMID: 25126958

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156723 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA replication regulator SLD3 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 292 amino acids
Theoretical weight: 34.24 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53135 (Residues: 148-430; Coverage: 42%)
Gene names: G2980, SLD3, YGL113W
Sequence domains: DNA replication regulator SLD3, STD domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 24 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 65.264Å b: 92.82Å c: 160.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.262
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Switch on the engine: how the eukaryotic replicative helicase MCM2-7 becomes activated.
Tognetti et al. (2015)
3 more