› 3wn1

X-ray diffraction
2Å resolution

Crystal Structure of Streptomyces coelicolor alpha-L-arabinofuranosidase in complex with xylotriose

Released:
DOI: 10.2210/pdb3wn1/pdb
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PDB entry 3wn1 coloured by chain, front view.
PDB entry 3wn1 coloured by chain, side view.
PDB entry 3wn1 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Crystal structure and characterization of the glycoside hydrolase family 62 α-L-arabinofuranosidase from Streptomyces coelicolor.
Maehara T, Fujimoto Z, Ichinose H, Michikawa M, Harazono K, Kaneko S
J Biol Chem 289 7962-72 (2014)
PMID: 24482228

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Extracellular exo-alpha-L-arabinofuranosidase Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 46.52 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Streptomyces lividans
UniProt:
  • Canonical: O54161 (Residues: 38-475; Coverage: 100%)
Gene names: SC7H1.02, SCO5932, abfB
Sequence domains:
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
4 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand TRS 1 x TRS
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41212
Unit cell:
a: 97.668Å b: 97.668Å c: 104.019Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.204
Expression system: Streptomyces lividans

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Citations

2 review citations

GH62 arabinofuranosidases: Structure, function and applications.
Wilkens et al. (2017)
1 more

1 mention without citation

Crystal structure and characterization of the glycoside hydrolase family 62 α-L-arabinofuranosidase from Streptomyces coelicolor.
Maehara et al. (2014)

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