› 3wr5

X-ray diffraction
2.14Å resolution

Structural basis on the efficient CO2 reduction of acidophilic formate dehydrogenase

Released:
DOI: 10.2210/pdb3wr5/pdb
PDB entry 3wr5 coloured by chain, front view.
PDB entry 3wr5 coloured by chain, side view.
PDB entry 3wr5 coloured by chain, top view.
Source organism: Thiobacillus sp. KNK65MA
Entry authors: Ha JM, Jeon ST, Yoon HJ, Lee HH

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 406 amino acids
Theoretical weight: 44.48 KDa
Source organism: Thiobacillus sp. KNK65MA
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76EB7 (Residues: 1-401; Coverage: 100%)
Gene name: fdh65MA
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NAD 3 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 55.942Å b: 72.729Å c: 386.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.208
Expression system: Escherichia coli

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