3wrk

X-ray diffraction
2.61Å resolution

Crystal structure of P450cam

Released:
Model geometry
Fit model/data
Source organism: Pseudomonas putida
Entry authors: Kishimoto A, Takagi K, Amano A, Sakurai K, Mizushima T, Shimada H

Function and Biology Details

Reaction catalysed:
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chains: A, D
Molecule details ›
Chains: A, D
Length: 421 amino acids
Theoretical weight: 47.55 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 60.339Å b: 93.188Å c: 73.828Å
α: 90° β: 89.92° γ: 90°
R-values:
R R work R free
0.177 0.174 0.235
Expression system: Escherichia coli