› 3wt1

X-ray diffraction
1.85Å resolution

Crystal structure of the b'-a' domain of thermophilic fungal protein disulfide isomerase (reduced form)

Released:
DOI: 10.2210/pdb3wt1/pdb
PDB entry 3wt1 coloured by chain, front view.
PDB entry 3wt1 coloured by chain, side view.
PDB entry 3wt1 coloured by chain, top view.
Source organism: Humicola insolens
Primary publication:
Redox-dependent conformational transition of catalytic domain of protein disulfide isomerase indicated by crystal structure-based molecular dynamics simulation
Inagaki K, Satoh T, Itoh SG, Okumura H, Kato K
Chem Phys Lett 618 203-207 (2015)

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein disulfide-isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 27.01 KDa
Source organism: Humicola insolens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55059 (Residues: 228-469; Coverage: 50%)
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P1
Unit cell:
a: 65.77Å b: 67.75Å c: 70.25Å
α: 105.92° β: 113.13° γ: 96.59°
R-values:
R R work R free
0.24 0.238 0.278
Expression system: Escherichia coli

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