› 3wwl

X-ray diffraction
1.2Å resolution

Crystal structure of lysine biosynthetic amino acid carrier protein LysW from Thermus thermophilus conjugated with alpha-aminoadipate

Released:
DOI: 10.2210/pdb3wwl/pdb
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PDB entry 3wwl coloured by chain, front view.
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Source organism: Thermus thermophilus HB27
Primary publication:
Structural insight into amino group-carrier protein-mediated lysine biosynthesis: crystal structure of the LysZ·LysW complex from Thermus thermophilus.
Yoshida A, Tomita T, Fujimura T, Nishiyama C, Kuzuyama T, Nishiyama M
J Biol Chem 290 435-47 (2015)
PMID: 25392000

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-aminoadipate carrier protein LysW Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 5.96 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SH22 (Residues: 1-54; Coverage: 100%)
Gene names: TTHA1908, lysW
Sequence domains: TFIIB zinc-binding
Structure domains: Rubrerythrin, domain 2

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand R0K 1 x R0K

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P3221
Unit cell:
a: 48.932Å b: 48.932Å c: 38.654Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.145 0.155
Expression system: Escherichia coli

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Citations

1 review citation

Biosynthesis of Oligopeptides Using ATP-Grasp Enzymes.
Ogasawara et al. (2017)
6 other articles

3 mentions without citation

Lysine Biosynthesis of Thermococcus kodakarensis with the Capacity to Function as an Ornithine Biosynthetic System.
Yoshida et al. (2016)
2 more

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