› 3x0k

X-ray diffraction
0.97Å resolution

ADP ribose pyrophosphatase from Thermus thermophilus HB8 in ES-state at 0.97 angstrom resolution

Released:
DOI: 10.2210/pdb3x0k/pdb
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Source organism: Thermus thermophilus HB8
Primary publication:
ADP-Ribose Pyrophosphatase Reaction in Crystalline State Conducted by Consecutive Binding of Two Manganese(II) Ions as Cofactors.
Furuike Y, Akita Y, Miyahara I, Kamiya N
Biochemistry 55 1801-12 (2016)
PMID: 26979298

Function and Biology Details

Reaction catalysed: 
ADP-D-ribose + H(2)O = AMP + D-ribose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nudix hydrolase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.29 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SKW5 (Residues: 1-170; Coverage: 100%)
Gene name: TTHA0528
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

2 bound ligands:
Ligand AR6 1 x AR6
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3221
Unit cell:
a: 49.645Å b: 49.645Å c: 118.673Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.131 not available not available
Expression system: Escherichia coli

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Citations

3 citation in other articles

Visualization of mutagenic nucleotide processing by Escherichia coli MutT, a Nudix hydrolase.
Nakamura et al. (2022)
2 more

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