› 3zfc

X-ray diffraction
1.8Å resolution

Crystal Structure of the Kif4 Motor Domain Complexed With Mg-AMPPNP

Released:
DOI: 10.2210/pdb3zfc/pdb
PDB entry 3zfc coloured by chain, front view.
PDB entry 3zfc coloured by chain, side view.
PDB entry 3zfc coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for the ATP-induced isomerization of kinesin.
Chang Q, Nitta R, Inoue S, Hirokawa N
J Mol Biol 425 1869-1880 (2013)
PMID: 23500491

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosome-associated kinesin KIF4 Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 38.93 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33174 (Residues: 1-344; Coverage: 28%)
Gene names: Kif4, Kif4a, Kns4
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P41212
Unit cell:
a: 61.952Å b: 61.952Å c: 166.367Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Prime movers: the mechanochemistry of mitotic kinesins.
Cross et al. (2014)
2 more

4 mentions without citation

High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation.
Shang et al. (2014)
3 more