› 3zhm

X-ray diffraction
2.6Å resolution

N-terminal domain of the CI repressor from bacteriophage TP901-1 in complex with the OL2 operator half-site

Released:
DOI: 10.2210/pdb3zhm/pdb
PDB entry 3zhm coloured by chain, front view.
PDB entry 3zhm coloured by chain, side view.
PDB entry 3zhm coloured by chain, top view.
Source organism: Lactococcus phage TP901-1
Primary publication:
Binding of the N-terminal domain of the lactococcal bacteriophage TP901-1 CI repressor to its target DNA: a crystallography, small angle scattering, and nuclear magnetic resonance study.
Frandsen KH, Rasmussen KK, Jensen MR, Hammer K, Pedersen M, Poulsen JC, Arleth L, Lo Leggio L
Biochemistry 52 6892-904 (2013)
PMID: 24047404

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117116 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 9.11 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Escherichia coli
UniProt:
  • Canonical: O48503 (Residues: 2-74; Coverage: 41%)
Gene name: cI
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
5'-D(*AP*GP*TP*TP*CP*AP*CP*GP*TP*TP*CP*AP*AP*GP *TP*GP*CP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Not provided
5'-D(*AP*CP*GP*TP*GP*AP*AP*CP*TP*TP*GP*CP*AP*CP *TP*TP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Lactococcus phage TP901-1
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 29.86Å b: 64.07Å c: 67.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.218
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 citation in other articles

Structural and dynamics studies of a truncated variant of CI repressor from bacteriophage TP901-1.
Rasmussen et al. (2016)
5 more

2 mentions without citation

Evidence-Based Structural Model of the Staphylococcal Repressor Protein: Separation of Functions into Different Domains.
Nyíri et al. (2015)
1 more