› 3zir

X-ray diffraction
2.3Å resolution

minor-site specific NLS (B141)

Released:
DOI: 10.2210/pdb3zir/pdb
PDB entry 3zir coloured by chain, front view.
PDB entry 3zir coloured by chain, side view.
PDB entry 3zir coloured by chain, top view.
Source organisms:
Primary publication:
Distinctive conformation of minor site-specific nuclear localization signals bound to importin-α.
Chang CW, Couñago RM, Williams SJ, Bodén M, Kobe B
Traffic 14 1144-54 (2013)
PMID: 23910026

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-546857 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 49.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
B141NLS Chains: B, C
Molecule details ›
Chains: B, C
Length: 11 amino acids
Theoretical weight: 1.5 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 79Å b: 90.34Å c: 100.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
2 more

3 mentions without citation

FlexPepDock lessons from CAPRI peptide-protein rounds and suggested new criteria for assessment of model quality and utility.
Marcu et al. (2017)
2 more