› 3zjc

X-ray diffraction
3.15Å resolution

Crystal structure of GMPPNP-bound human GIMAP7 L100Q variant

Released:
DOI: 10.2210/pdb3zjc/pdb
PDB entry 3zjc coloured by chain, front view.
PDB entry 3zjc coloured by chain, side view.
PDB entry 3zjc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the mechanism of GTPase activation in the GIMAP family.
Schwefel D, Arasu BS, Marino SF, Lamprecht B, Köchert K, Rosenbaum E, Eichhorst J, Wiesner B, Behlke J, Rocks O, Mathas S, Daumke O
Structure 21 550-9 (2013)
PMID: 23454188

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase IMAP family member 7 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 305 amino acids
Theoretical weight: 34.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NHV1 (Residues: 1-300; Coverage: 100%)
Gene names: GIMAP7, IAN7
Sequence domains: AIG1 family

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand GNP 6 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P1
Unit cell:
a: 45.853Å b: 90.939Å c: 114.551Å
α: 77.3° β: 85.23° γ: 89.23°
R-values:
R R work R free
0.238 0.234 0.305
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Invited review: Mechanisms of GTP hydrolysis and conformational transitions in the dynamin superfamily.
Daumke et al. (2016)
2 more

5 mentions without citation

AIG1 affects in vitro and in vivo virulence in clinical isolates of Entamoeba histolytica.
Nakada-Tsukui et al. (2018)
4 more