› 3zka

X-ray diffraction
1.55Å resolution

CRYSTAL STRUCTURE OF PNEUMOCOCCAL SURFACE ANTIGEN PSAA D280N IN THE METAL-BOUND, OPEN STATE

Released:
DOI: 10.2210/pdb3zka/pdb
PDB entry 3zka coloured by chain, front view.
PDB entry 3zka coloured by chain, side view.
PDB entry 3zka coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Imperfect coordination chemistry facilitates metal ion release in the Psa permease.
Couñago RM, Ween MP, Begg SL, Bajaj M, Zuegg J, O'Mara ML, Cooper MA, McEwan AG, Paton JC, Kobe B, McDevitt CA
Nat Chem Biol 10 35-41 (2014)
PMID: 24212134

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-531751 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Manganese ABC transporter substrate-binding lipoprotein PsaA Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 31.44 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A4G2 (Residues: 32-309; Coverage: 96%)
Gene names: SP_1650, psaA
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 39.524Å b: 58.96Å c: 62.13Å
α: 106.18° β: 104.49° γ: 94.39°
R-values:
R R work R free
0.167 0.165 0.198
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Streptococcus pneumoniae: transmission, colonization and invasion.
Weiser et al. (2018)
20 more

1 mention without citation

Dysregulation of transition metal ion homeostasis is the molecular basis for cadmium toxicity in Streptococcus pneumoniae.
Begg et al. (2015)