› 3zuy

X-ray diffraction
2.2Å resolution

Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.

Released:
DOI: 10.2210/pdb3zuy/pdb
PDB entry 3zuy coloured by chain, front view.
PDB entry 3zuy coloured by chain, side view.
PDB entry 3zuy coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
Hu NJ, Iwata S, Cameron AD, Drew D
OpenAccess logo Nature 478 408-11 (2011)
PMID: 21976025

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575482 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transporter Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 33.9 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9K0A9 (Residues: 1-315; Coverage: 100%)
Gene name: NMB0705
Sequence domains: Sodium Bile acid symporter family
Structure domains: Na+/H+ antiporter like fold

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand TCH 2 x TCH
Ligand LDA 5 x LDA
Ligand PTY 2 x PTY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P4122
Unit cell:
a: 73.35Å b: 73.35Å c: 162.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

37 review citations

The ABCs of membrane transporters in health and disease (SLC series): introduction.
Hediger et al. (2013)
36 more

21 mentions without citation

The solute carrier family 10 (SLC10): beyond bile acid transport.
Claro da Silva et al. (2013)
20 more