› 3zx6

X-ray diffraction
2.65Å resolution

Structure of Hamp(AF1503)-Tsr fusion - Hamp (A291V) mutant

Released:
DOI: 10.2210/pdb3zx6/pdb
Model geometry
Fit model/data
PDB entry 3zx6 coloured by chain, front view.
PDB entry 3zx6 coloured by chain, side view.
PDB entry 3zx6 coloured by chain, top view.
Source organisms:
Primary publication:
Axial helix rotation as a mechanism for signal regulation inferred from the crystallographic analysis of the E. coli serine chemoreceptor.
Ferris HU, Zeth K, Hulko M, Dunin-Horkawicz S, Lupas AN
J Struct Biol 186 349-56 (2014)
PMID: 24680785

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128060 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyl-accepting chemotaxis protein I; histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 341 amino acids
Theoretical weight: 36.14 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28769 (Residues: 278-326; Coverage: 15%)
  • Canonical: P02942 (Residues: 264-518, 519-551; Coverage: 52%)
Gene names: AF_1503, JW4318, b4355, cheD, tsr
Sequence domains:
Structure domains: Methyl-accepting chemotaxis protein

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 112.22Å b: 80.02Å c: 139.17Å
α: 90° β: 110.78° γ: 90°
R-values:
R R work R free
0.231 0.229 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Signaling and sensory adaptation in Escherichia coli chemoreceptors: 2015 update.
Parkinson et al. (2015)
9 more

10 mentions without citation

Decoding the chemotactic signal.
Thomas et al. (2018)
9 more