› 3zy3

X-ray diffraction
1.86Å resolution

Crystal structure of POFUT1 in complex with GDP (crystal-form-III)

Released:
DOI: 10.2210/pdb3zy3/pdb
PDB entry 3zy3 coloured by chain, front view.
PDB entry 3zy3 coloured by chain, side view.
PDB entry 3zy3 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural insights into the mechanism of protein O-fucosylation.
Lira-Navarrete E, Valero-González J, Villanueva R, Martínez-Júlvez M, Tejero T, Merino P, Panjikar S, Hurtado-Guerrero R
OpenAccess logo PLoS One 6 e25365 (2011)
PMID: 21966509

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + [protein]-(L-serine/L-threonine) = GDP + [protein]-3-O-(alpha-L-fucosyl)-(L-serine/L-threonine)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-fucose protein O-fucosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 41.01 KDa
Source organism: Caenorhabditis elegans
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q18014 (Residues: 26-383; Coverage: 97%)
Gene names: C15C7.7, pfut-1
Sequence domains: GDP-fucose protein O-fucosyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P65
Unit cell:
a: 132.18Å b: 132.18Å c: 77.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.245
Expression system: Komagataella pastoris

Quick links

Citations

12 review citations

The Canonical Notch Signaling Pathway: Structural and Biochemical Insights into Shape, Sugar, and Force.
Kovall et al. (2017)
11 more

3 mentions without citation

A perspective on structural and mechanistic aspects of protein O-fucosylation.
Lira-Navarrete et al. (2018)
2 more