› 3zzk

X-ray diffraction
1.78Å resolution

Structure of an engineered aspartate aminotransferase

Released:
DOI: 10.2210/pdb3zzk/pdb
PDB entry 3zzk coloured by chain, front view.
PDB entry 3zzk coloured by chain, side view.
PDB entry 3zzk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and mechanism of a cysteine sulfinate desulfinase engineered on the aspartate aminotransferase scaffold.
Fernandez FJ, de Vries D, Peña-Soler E, Coll M, Christen P, Gehring H, Vega MC
Biochim Biophys Acta 1824 339-49 (2012)
PMID: 22138634

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00509 (Residues: 1-396; Coverage: 100%)
Gene names: JW0911, aspC, b0928
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 1 x PMP
2 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 85.13Å b: 154.34Å c: 79.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.199
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

A novel 3-sulfinopropionyl coenzyme A (3SP-CoA) desulfinase from Advenella mimigardefordensis strain DPN7T acting as a key enzyme during catabolism of 3,3'-dithiodipropionic acid is a member of the acyl-CoA dehydrogenase superfamily.
Schürmann et al. (2013)
8 more