› 3zzz

X-ray diffraction
1.55Å resolution

Crystal structure of a Raver1 PRI4 peptide in complex with polypyrimidine tract binding protein RRM2

Released:
DOI: 10.2210/pdb3zzz/pdb
PDB entry 3zzz coloured by chain, front view.
PDB entry 3zzz coloured by chain, side view.
PDB entry 3zzz coloured by chain, top view.
Source organisms:
Primary publication:
Crystallographic analysis of polypyrimidine tract-binding protein-Raver1 interactions involved in regulation of alternative splicing.
Joshi A, Coelho MB, Kotik-Kogan O, Simpson PJ, Matthews SJ, Smith CW, Curry S
OpenAccess logo Structure 19 1816-25 (2011)
PMID: 22153504

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150780 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polypyrimidine tract-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26599 (Residues: 156-285; Coverage: 23%)
Gene names: PTB, PTBP1
Sequence domains: RRM-like domain
Structure domains: Alpha-Beta Plaits
Ribonucleoprotein PTB-binding 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 amino acids
Theoretical weight: 1.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CW46 (Residues: 680-692; Coverage: 2%)
Gene names: Kiaa1978, Raver1

Ligands and Environments

1 bound ligand:
Ligand IOD 5 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 74.48Å b: 60.38Å c: 61.06Å
α: 90° β: 107.86° γ: 90°
R-values:
R R work R free
0.217 0.217 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

New insights into functional roles of the polypyrimidine tract-binding protein.
Romanelli et al. (2013)
4 more

1 mention without citation

Crystallographic analysis of polypyrimidine tract-binding protein-Raver1 interactions involved in regulation of alternative splicing.
Joshi et al. (2011)