› 435d

X-ray diffraction
1.4Å resolution

5'-R(*UP*AP*GP*CP*CP*CP*C)-3', 5'-R(*GP*GP*GP*GP*CP*UP*A)-3'

Released:
DOI: 10.2210/pdb435d/pdb
PDB entry 435d coloured by chain, front view.
PDB entry 435d coloured by chain, side view.
PDB entry 435d coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystal structure of acceptor stem of tRNA(Ala) from Escherichia coli shows unique G.U wobble base pair at 1.16 A resolution.
Mueller U, Schübel H, Sprinzl M, Heinemann U
RNA 5 670-7 (1999)
PMID: 10334337

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196769 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (5'-R(*UP*AP*GP*CP*CP*CP*C)-3') Chains: A, C
Molecule details ›
Chains: A, C
Length: 7 nucleotides
Theoretical weight: 2.16 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(*GP*GP*GP*GP*CP*UP*A)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 7 nucleotides
Theoretical weight: 2.28 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P1
Unit cell:
a: 26.68Å b: 26.697Å c: 30.459Å
α: 104.29° β: 104.22° γ: 91.66°
R-values:
R R work R free
0.161 0.159 0.218
Expression system: Not provided

Quick links

Citations

9 review citations

The G x U wobble base pair. A fundamental building block of RNA structure crucial to RNA function in diverse biological systems.
Varani et al. (2000)
8 more

1 mention without citation

The electrostatic characteristics of G.U wobble base pairs.
Xu et al. (2007)