› 4a4m

X-ray diffraction
3.3Å resolution

Crystal structure of the light-activated constitutively active N2C, M257Y,D282C rhodopsin mutant in complex with a peptide resembling the C-terminus of the Galpha-protein subunit (GaCT)

Released:
DOI: 10.2210/pdb4a4m/pdb
PDB entry 4a4m coloured by chain, front view.
PDB entry 4a4m coloured by chain, side view.
PDB entry 4a4m coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II.
Deupi X, Edwards P, Singhal A, Nickle B, Oprian D, Schertler G, Standfuss J
Proc Natl Acad Sci U S A 109 119-24 (2012)
PMID: 22198838

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136078 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.07 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.27 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P04695 (Residues: 340-350; Coverage: 3%)
Gene name: GNAT1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand RET 1 x RET
Ligand ACT 1 x ACT
Ligand BOG 1 x BOG
Ligand PLM 1 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 242.19Å b: 242.19Å c: 109.829Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.262
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

39 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
38 more

30 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
29 more