› 4aa8

X-ray diffraction
1.8Å resolution

Bovine chymosin at 1.8A resolution

Released:
DOI: 10.2210/pdb4aa8/pdb
PDB entry 4aa8 coloured by chain, front view.
PDB entry 4aa8 coloured by chain, side view.
PDB entry 4aa8 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Camel and bovine chymosin: the relationship between their structures and cheese-making properties.
Langholm Jensen J, Mølgaard A, Navarro Poulsen JC, Harboe MK, Simonsen JB, Lorentzen AM, Hjernø K, van den Brink JM, Qvist KB, Larsen S
OpenAccess logo Acta Crystallogr D Biol Crystallogr 69 901-13 (2013)
PMID: 23633601

Function and Biology Details

Reaction catalysed: 
Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chymosin Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.67 KDa
Source organism: Bos taurus
Expression system: Aspergillus niger
UniProt:
  • Canonical: P00794 (Residues: 59-381; Coverage: 89%)
Gene names: CPC, CYM
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

1 bound ligand:
Ligand CL 8 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: I222
Unit cell:
a: 71.61Å b: 79.17Å c: 113.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.215
Expression system: Aspergillus niger

Quick links

Citations

3 review citations

A Review on Processing Opportunities for the Development of Camel Dairy Products.
Arain et al. (2023)
2 more

2 mentions without citation

Camel and bovine chymosin: the relationship between their structures and cheese-making properties.
Langholm Jensen et al. (2013)
1 more