› 4ac6

X-ray diffraction
2.54Å resolution

Corynebacterium glutamicum AcnR AU derivative structure

Released:
DOI: 10.2210/pdb4ac6/pdb
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PDB entry 4ac6 coloured by chain, front view.
PDB entry 4ac6 coloured by chain, side view.
PDB entry 4ac6 coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
Crystal and solution studies reveal that the transcriptional regulator AcnR of Corynebacterium glutamicum is regulated by citrate-Mg2+ binding to a non-canonical pocket.
García-Nafría J, Baumgart M, Turkenburg JP, Wilkinson AJ, Bott M, Wilson KS
J Biol Chem 288 15800-12 (2013)
PMID: 23589369

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional repressor AcnR Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.51 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NQ97 (Residues: 2-188; Coverage: 100%)
Gene names: Cgl1541, acnR, cg1738
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

1 bound ligand:
Ligand AU 4 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 34.142Å b: 72.886Å c: 73.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.263
Expression system: Escherichia coli

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Citations

6 citation in other articles

IpsA, a novel LacI-type regulator, is required for inositol-derived lipid formation in Corynebacteria and Mycobacteria.
Baumgart et al. (2013)
5 more

2 mentions without citation

The TetR family of regulators.
Cuthbertson et al. (2013)
1 more

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