› 4auc

X-ray diffraction
1.6Å resolution

Bovine chymosin in complex with Pepstatin A

Released:
DOI: 10.2210/pdb4auc/pdb
PDB entry 4auc coloured by chain, front view.
PDB entry 4auc coloured by chain, side view.
PDB entry 4auc coloured by chain, top view.
Source organisms:
Entry authors: Langholm Jensen J, Navarro Poulsen JC, Jacobsen J, van den Brink JM, Qvist KB, Larsen S

Function and Biology Details

Reaction catalysed: 
Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-524332 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chymosin Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 35.67 KDa
Source organism: Bos taurus
Expression system: Aspergillus niger
UniProt:
  • Canonical: P00794 (Residues: 59-381; Coverage: 89%)
Gene names: CPC, CYM
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases
PEPSTATIN A Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 686 Da
Source organism: Actinomyces

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand CD 1 x CD
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3221
Unit cell:
a: 93.5Å b: 93.5Å c: 89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.197
Expression system: Aspergillus niger

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