› 4bez

X-ray diffraction
3.3Å resolution

Night blindness causing G90D rhodopsin in the active conformation

Released:
DOI: 10.2210/pdb4bez/pdb
PDB entry 4bez coloured by chain, front view.
PDB entry 4bez coloured by chain, side view.
PDB entry 4bez coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Insights into congenital stationary night blindness based on the structure of G90D rhodopsin.
Singhal A, Ostermaier MK, Vishnivetskiy SA, Panneels V, Homan KT, Tesmer JJ, Veprintsev D, Deupi X, Gurevich VV, Schertler GF, Standfuss J
EMBO Rep 14 520-6 (2013)
PMID: 23579341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136077 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.09 KDa
Source organism: Bos taurus
Expression system: Homo sapiens
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand ACT 1 x ACT
Ligand SO4 1 x SO4
Ligand PLM 1 x PLM
Ligand BOG 1 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 242.44Å b: 242.44Å c: 110.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.229 0.244
Expression system: Homo sapiens

Quick links

Citations

15 review citations

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
14 more

8 mentions without citation

Structural Basis for G Protein-Coupled Receptor Activation.
Manglik et al. (2017)
7 more