› 4bqu

X-ray diffraction
2.36Å resolution

Japanin from Rhipicephalus appendiculatus bound to cholesterol: Orthorhombic crystal form

Released:
DOI: 10.2210/pdb4bqu/pdb
PDB entry 4bqu coloured by chain, front view.
PDB entry 4bqu coloured by chain, side view.
PDB entry 4bqu coloured by chain, top view.
Source organism: Rhipicephalus appendiculatus
Primary publication:
Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.
Roversi P, Johnson S, Preston SG, Nunn MA, Paesen GC, Austyn JM, Nuttall PA, Lea SM
OpenAccess logo Sci Rep 7 16057 (2017)
PMID: 29167574

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Japanin Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 18.64 KDa
Source organism: Rhipicephalus appendiculatus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: M1MR49 (Residues: 25-176; Coverage: 100%)
Structure domains: Lipocalin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand CLR 2 x CLR
Ligand EDO 7 x EDO
Ligand NAG 1 x NAG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 80.18Å b: 133.6Å c: 71.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.253
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

The Use of Tick Salivary Proteins as Novel Therapeutics.
Chmelař et al. (2019)
3 more

1 mention without citation

Structural basis of cholesterol binding by a novel clade of dendritic cell modulators from ticks.
Roversi et al. (2017)