› 4bsb

X-ray diffraction
2.35Å resolution

Human H7N9 Influenza Virus Haemagglutinin (with Asn-133 Glycosylation) in Complex with Human Receptor Analogue LSTc

Released:
DOI: 10.2210/pdb4bsb/pdb
PDB entry 4bsb coloured by chain, front view.
PDB entry 4bsb coloured by chain, side view.
PDB entry 4bsb coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Receptor binding by an H7N9 influenza virus from humans.
Xiong X, Martin SR, Haire LF, Wharton SA, Daniels RS, Bennett MS, McCauley JW, Collins PJ, Walker PA, Skehel JJ, Gamblin SJ
Nature 499 496-9 (2013)
PMID: 23787694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126034 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.04 KDa
Source organism: Influenza A virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: M4YV75 (Residues: 19-339; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.44 KDa
Source organism: Influenza A virus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: M4YV75 (Residues: 340-516; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 4 x NAG
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R32
Unit cell:
a: 116.348Å b: 116.348Å c: 296.516Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.228 0.265
Expression system: Spodoptera frugiperda

Quick links

Citations

51 review citations

Host and viral determinants of influenza A virus species specificity.
Long et al. (2019)
50 more

3 mentions without citation

New insights into influenza A specificity: an evolution of paradigms.
Ji et al. (2017)
2 more