› 4c7n

X-ray diffraction
2.1Å resolution

Crystal Structure of the synthetic peptide iM10 in complex with the coiled-coil region of MITF

Released:
DOI: 10.2210/pdb4c7n/pdb
PDB entry 4c7n coloured by chain, front view.
PDB entry 4c7n coloured by chain, side view.
PDB entry 4c7n coloured by chain, top view.
Source organisms:
Primary publication:
Improving coiled coil stability while maintaining specificity by a bacterial hitchhiker selection system.
Kükenshöner T, Wohlwend D, Niemöller C, Dondapati P, Speck J, Adeniran AV, Nieth A, Gerhardt S, Einsle O, Müller KM, Arndt KM
J Struct Biol 186 335-48 (2014)
PMID: 24631970

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130819 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microphthalmia-associated transcription factor Chain: A
Molecule details ›
Chain: A
Length: 51 amino acids
Theoretical weight: 6.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O75030 (Residues: 356-403; Coverage: 9%)
Gene names: BHLHE32, MITF
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
SYNTHETIC ALPHA-HELIX, IM10 Chain: B
Molecule details ›
Chain: B
Length: 51 amino acids
Theoretical weight: 6.09 KDa
Source organism: synthetic construct
Expression system: Not provided
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand HG 6 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 23.701Å b: 33.265Å c: 61.125Å
α: 90° β: 94.65° γ: 90°
R-values:
R R work R free
0.229 0.228 0.253
Expression system: Not provided

Quick links

Citations

2 review citations

SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions.
Zhao et al. (2015)
1 more

3 mentions without citation

Designing helical peptide inhibitors of protein-protein interactions.
Rezaei Araghi et al. (2016)
2 more