› 4c8x

X-ray diffraction
2Å resolution

Crystal structure of carbohydrate-binding module CBM3b mutant (Y56S) from the cellulosomal cellobiohydrolase 9A from Clostridium thermocellum

Released:
DOI: 10.2210/pdb4c8x/pdb
PDB entry 4c8x coloured by chain, front view.
PDB entry 4c8x coloured by chain, side view.
PDB entry 4c8x coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Entry authors: Yaniv O, Bayer EA, Lamed R, Frolow F

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 145 amino acids
Theoretical weight: 16.46 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59325 (Residues: 1004-1147; Coverage: 12%)
Gene name: cbhA
Sequence domains: Cellulose binding domain
Structure domains: Endoglucanase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41
Unit cell:
a: 95.186Å b: 95.186Å c: 83.637Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.203
Expression system: Escherichia coli BL21(DE3)

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