› 4cdo

X-ray diffraction
2.5Å resolution

Crystal structure of PQBP1 bound to spliceosomal U5-15kD

Released:
DOI: 10.2210/pdb4cdo/pdb
PDB entry 4cdo coloured by chain, front view.
PDB entry 4cdo coloured by chain, side view.
PDB entry 4cdo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutations in the PQBP1 gene prevent its interaction with the spliceosomal protein U5-15 kD.
Mizuguchi M, Obita T, Serita T, Kojima R, Nabeshima Y, Okazawa H
Nat Commun 5 3822 (2014)
PMID: 24781215

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-521168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyglutamine-binding protein 1; Thioredoxin-like protein 4A Chains: A, C
Molecule details ›
Chains: A, C
Length: 185 amino acids
Theoretical weight: 21.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83876 (Residues: 4-138; Coverage: 95%)
  • Canonical: O60828 (Residues: 224-265; Coverage: 16%)
Gene names: DIM1, JM26, NPW38, PQBP1, TXNL4, TXNL4A
Sequence domains: Mitosis protein DIM1
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 59.36Å b: 53.7Å c: 66.19Å
α: 90° β: 89.95° γ: 90°
R-values:
R R work R free
0.19 0.187 0.238
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Ultra-Early Phase pathologies of Alzheimer's disease and other neurodegenerative diseases.
Okazawa H. (2017)
2 more