› 4dw0

X-ray diffraction
2.9Å resolution

Crystal structure of the ATP-gated P2X4 ion channel in the closed, apo state at 2.9 Angstroms

Released:
DOI: 10.2210/pdb4dw0/pdb
PDB entry 4dw0 coloured by chain, front view.
PDB entry 4dw0 coloured by chain, side view.
PDB entry 4dw0 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Molecular mechanism of ATP binding and ion channel activation in P2X receptors.
Hattori M, Gouaux E
Nature 485 207-12 (2012)
PMID: 22535247

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
P2X purinoceptor 4a Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.16 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: F8W463 (Residues: 28-365; Coverage: 87%)
Gene name: p2rx4a
Sequence domains: ATP P2X receptor
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand GD 1 x GD
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 99.626Å b: 99.626Å c: 441.539Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.22 0.244
Expression system: Spodoptera frugiperda

Quick links

Citations

94 review citations

Neuromodulation by extracellular ATP and P2X receptors in the CNS.
Khakh et al. (2012)
93 more

56 mentions without citation

P2X receptors as drug targets.
North et al. (2013)
55 more