› 4e84

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110019 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 38.74 KDa
Source organism: Burkholderia cenocepacia J2315
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EB35 (Residues: 1-316; Coverage: 100%)
Gene names: BCAL2945, hldA
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

7 bound ligands:
Ligand ANP 2 x ANP
Ligand M7B 1 x M7B
Ligand K 2 x K
Ligand CL 2 x CL
Ligand PO4 2 x PO4
Ligand MG 2 x MG
Ligand GMZ 1 x GMZ
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6122
Unit cell:
a: 90.398Å b: 90.398Å c: 397.917Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.263
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery.
Vivoli et al. (2015)
3 more