› 4en9

X-ray diffraction
2.64Å resolution

Crystal structure of HA70 (HA3) subcomponent of Clostridium botulinum type C progenitor toxin in complex with alpha 2-6-sialyllactosamine

Released:
DOI: 10.2210/pdb4en9/pdb
PDB entry 4en9 coloured by chain, front view.
PDB entry 4en9 coloured by chain, side view.
PDB entry 4en9 coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides.
Yamashita S, Yoshida H, Uchiyama N, Nakakita Y, Nakakita S, Tonozuka T, Oguma K, Nishikawa A, Kamitori S
FEBS Lett 586 2404-10 (2012)
PMID: 22684008

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-545784 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Hemagglutinin component HA-23A Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 26.05 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: P46085 (Residues: 1-203; Coverage: 33%)
Gene names: HA-70, antP-70, cha-70
Sequence domains: Clostridium enterotoxin
Structure domains: Proaerolysin; Chain A, domain 3
Hemagglutinin component HA-53 Chain: B
Molecule details ›
Chain: B
Length: 420 amino acids
Theoretical weight: 47.33 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: P46085 (Residues: 204-623; Coverage: 67%)
Gene names: HA-70, antP-70, cha-70
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand MRD 5 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P63
Unit cell:
a: 175.977Å b: 175.977Å c: 80.636Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.22
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Architecture of the botulinum neurotoxin complex: a molecular machine for protection and delivery.
Lam et al. (2015)
3 more

1 mention without citation

The human milk oligosaccharides 2'-fucosyllactose and 6'-sialyllactose protect against the development of necrotizing enterocolitis by inhibiting toll-like receptor 4 signaling.
Sodhi et al. (2021)