› 4fft

X-ray diffraction
2.1Å resolution

Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with mixed-linkage glucan

Released:
DOI: 10.2210/pdb4fft/pdb
PDB entry 4fft coloured by chain, front view.
PDB entry 4fft coloured by chain, side view.
PDB entry 4fft coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural basis for entropy-driven cellulose binding by a type-A cellulose-binding module (CBM) and bacterial expansin.
Georgelis N, Yennawar NH, Cosgrove DJ
Proc Natl Acad Sci U S A 109 14830-5 (2012)
PMID: 22927418

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Expansin-YoaJ Chains: A, B
Molecule details ›
Chains: A, B
Length: 208 amino acids
Theoretical weight: 23.1 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O34918 (Residues: 26-232; Coverage: 100%)
Gene names: BSU18630, yoaJ
Sequence domains: Lytic transglycolase
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
2 bound ligands:
Ligand SO4 1 x SO4
Ligand ACY 4 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P31
Unit cell:
a: 56.562Å b: 56.562Å c: 146.104Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.229
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Xyloglucan and its interactions with other components of the growing cell wall.
Park et al. (2015)
17 more

2 mentions without citation

Structural basis for entropy-driven cellulose binding by a type-A cellulose-binding module (CBM) and bacterial expansin.
Georgelis et al. (2012)
1 more