› 4fqg

X-ray diffraction
2Å resolution

Crystal structure of the TCERG1 FF4-6 tandem repeat domain

Released:
DOI: 10.2210/pdb4fqg/pdb
PDB entry 4fqg coloured by chain, front view.
PDB entry 4fqg coloured by chain, side view.
PDB entry 4fqg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Specific interaction of the transcription elongation regulator TCERG1 with RNA polymerase II requires simultaneous phosphorylation at Ser2, Ser5, and Ser7 within the carboxyl-terminal domain repeat.
Liu J, Fan S, Lee CJ, Greenleaf AL, Zhou P
J Biol Chem 288 10890-901 (2013)
PMID: 23436654

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127124 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation regulator 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14776 (Residues: 895-1081; Coverage: 17%)
Gene names: CA150, TAF2S, TCERG1
Sequence domains: FF domain
Structure domains: FF domain

Ligands and Environments

2 bound ligands:
Ligand NI 2 x NI
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 27.919Å b: 77.087Å c: 95.179Å
α: 90° β: 96.01° γ: 90°
R-values:
R R work R free
0.192 0.189 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain.
Harlen et al. (2017)
7 more

4 mentions without citation

What's all the phos about? Insights into the phosphorylation state of the RNA polymerase II C-terminal domain via mass spectrometry.
LeBlanc et al. (2021)
3 more