› 4he7

X-ray diffraction
1.8Å resolution

Crystal Structure of Brazzein

Released:
DOI: 10.2210/pdb4he7/pdb
PDB entry 4he7 coloured by chain, front view.
PDB entry 4he7 coloured by chain, side view.
PDB entry 4he7 coloured by chain, top view.
Source organism: Pentadiplandra brazzeana
Primary publication:
The structure of brazzein, a sweet-tasting protein from the wild African plant Pentadiplandra brazzeana.
Nagata K, Hongo N, Kameda Y, Yamamura A, Sasaki H, Lee WC, Ishikawa K, Suzuki E, Tanokura M
Acta Crystallogr D Biol Crystallogr 69 642-7 (2013)
PMID: 23519673

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Defensin-like protein Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 6.49 KDa
Source organism: Pentadiplandra brazzeana
UniProt:
  • Canonical: P56552 (Residues: 1-54; Coverage: 100%)
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I4122
Unit cell:
a: 61.4Å b: 61.4Å c: 59.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.252

Quick links

Citations

1 review citation

Brazzein and Monellin: Chemical Analysis, Food Industry Applications, Safety and Quality Control, Nutritional Profile and Health Impacts.
Saraiva et al. (2023)
2 other articles

2 mentions without citation

Structure-function relationships of brazzein variants with altered interactions with the human sweet taste receptor.
Singarapu et al. (2016)
1 more