› 4hij

X-ray diffraction
2.1Å resolution

Anti-Streptococcus pneumoniae 23F Fab 023.102 with bound L-rhamnose-(1-2)-alpha-D-galactose-(3-O)-phosphate-2-glycerol

Released:
DOI: 10.2210/pdb4hij/pdb
PDB entry 4hij coloured by chain, front view.
PDB entry 4hij coloured by chain, side view.
PDB entry 4hij coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of Preferred Human IgV Genes-Based Protective Antibodies Identify How Conserved Residues Contact Diverse Antigens and Assign Source of Specificity to CDR3 Loop Variation.
Bryson S, Thomson CA, Risnes LF, Dasgupta S, Smith K, Schrader JW, Pai EF
J Immunol 196 4723-30 (2016)
PMID: 27183571

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213493 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab 023.102 light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 219 amino acids
Theoretical weight: 23.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab 023.102 heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 231 amino acids
Theoretical weight: 25.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: MBG, RAM
Carbohydrate polymer
2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 143.87Å b: 66.488Å c: 118.214Å
α: 90° β: 111.58° γ: 90°
R-values:
R R work R free
0.249 0.249 0.262
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Individual and Population Genetics of Antibody Immunity.
Watson et al. (2017)
2 more

1 mention without citation

Human monoclonal antibodies isolated from a primary pneumococcal conjugate Vaccinee demonstrates the expansion of an antigen-driven Hypermutated memory B cell response.
Chen et al. (2018)