› 4hln

X-ray diffraction
2.7Å resolution

Structure of barley starch synthase I in complex with maltooligosaccharide

Released:
DOI: 10.2210/pdb4hln/pdb
PDB entry 4hln coloured by chain, front view.
PDB entry 4hln coloured by chain, side view.
PDB entry 4hln coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
Structure of starch synthase I from barley: insight into regulatory mechanisms of starch synthase activity.
Cuesta-Seijo JA, Nielsen MM, Marri L, Tanaka H, Beeren SR, Palcic MM
Acta Crystallogr D Biol Crystallogr 69 1013-25 (2013)
PMID: 23695246

Function and Biology Details

Reaction catalysed: 
ADP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = ADP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Starch synthase, chloroplastic/amyloplastic Chain: A
Molecule details ›
Chain: A
Length: 633 amino acids
Theoretical weight: 69.9 KDa
Source organism: Hordeum vulgare
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9M5A3 (Residues: 32-643; Coverage: 95%)
Gene name: SSI
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 153.15Å b: 153.15Å c: 84.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.173 0.198
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Formation of starch in plant cells.
Pfister et al. (2016)
2 more

5 mentions without citation

Potato starch synthases: Functions and relationships.
Nazarian-Firouzabadi et al. (2017)
4 more