› 4hlz

X-ray diffraction
2.9Å resolution

Crystal Structure of Fab C179 in Complex with a H2N2 influenza virus hemagglutinin

Released:
DOI: 10.2210/pdb4hlz/pdb
PDB entry 4hlz coloured by chain, front view.
PDB entry 4hlz coloured by chain, side view.
PDB entry 4hlz coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a classical broadly neutralizing stem antibody in complex with a pandemic H2 influenza virus hemagglutinin.
Dreyfus C, Ekiert DC, Wilson IA
J Virol 87 7149-54 (2013)
PMID: 23552413

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-504496 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
Hemagglutinin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 327 amino acids
Theoretical weight: 36.5 KDa
Source organism: Influenza A virus (A/Japan/305/1957(H2N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C7S226 (Residues: 15-340; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 174 amino acids
Theoretical weight: 20.14 KDa
Source organism: Influenza A virus (A/Japan/305/1957(H2N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C7S226 (Residues: 341-514; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Fab C179 heavy chain Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 229 amino acids
Theoretical weight: 24.85 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins
Fab C179 light chain Chains: H, J, L
Molecule details ›
Chains: H, J, L
Length: 214 amino acids
Theoretical weight: 23.48 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 5 x NAG
Ligand SO4 7 x SO4
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 136.58Å b: 150.78Å c: 217.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.284
Expression system: Trichoplusia ni

Quick links

Citations

20 review citations

Next-generation influenza vaccines: opportunities and challenges.
Wei et al. (2020)
19 more

21 mentions without citation

Influenza virus hemagglutinin stalk-based antibodies and vaccines.
Krammer et al. (2013)
20 more