› 4jpw

X-ray diffraction
2.9Å resolution

Crystal structure of broadly and potently neutralizing antibody 12a21 in complex with hiv-1 strain 93th057 gp120 mutant

Released:
DOI: 10.2210/pdb4jpw/pdb
PDB entry 4jpw coloured by chain, front view.
PDB entry 4jpw coloured by chain, side view.
PDB entry 4jpw coloured by chain, top view.
Source organisms:
Primary publication:
Somatic mutations of the immunoglobulin framework are generally required for broad and potent HIV-1 neutralization.
Klein F, Diskin R, Scheid JF, Gaebler C, Mouquet H, Georgiev IS, Pancera M, Zhou T, Incesu RB, Fu BZ, Gnanapragasam PN, Oliveira TY, Seaman MS, Kwong PD, Bjorkman PJ, Nussenzweig MC
Cell 153 126-38 (2013)
PMID: 23540694

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211972 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein gp160 Chain: G
Molecule details ›
Chain: G
Length: 353 amino acids
Theoretical weight: 39.16 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q0ED31 (Residues: 43-486; Coverage: 41%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
HEAVY CHAIN OF ANTIBODY 12A21 Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 24.41 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
LIGHT CHAIN OF ANTIBODY 12A21 Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NAG 10 x NAG
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 61.975Å b: 65.987Å c: 213.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.265
Expression system: Homo sapiens

Quick links

Citations

99 review citations

Germinal Center B Cell Dynamics.
Mesin et al. (2016)
98 more

11 mentions without citation

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design.
Burton et al. (2016)
10 more