› 4jtv

X-ray diffraction
3Å resolution

Crystal structure of 2009 pandemic influenza virus hemagglutinin complexed with human receptor analogue LSTc

Released:
DOI: 10.2210/pdb4jtv/pdb
PDB entry 4jtv coloured by chain, front view.
PDB entry 4jtv coloured by chain, side view.
PDB entry 4jtv coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Molecular basis of the receptor binding specificity switch of the hemagglutinins from both the 1918 and 2009 pandemic influenza A viruses by a D225G substitution.
Zhang W, Shi Y, Qi J, Gao F, Li Q, Fan Z, Yan J, Gao GF
J Virol 87 5949-58 (2013)
PMID: 23514882

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111242 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 321 amino acids
Theoretical weight: 35.72 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C3W5S1 (Residues: 18-338; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 162 amino acids
Theoretical weight: 18.56 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C3W5S1 (Residues: 345-506; Coverage: 29%)
Gene name: HA
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, SIA, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, SIA, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 7 x NAG
Ligand SIA 1 x SIA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 66.722Å b: 117.321Å c: 117.393Å
α: 61.78° β: 81.82° γ: 77.42°
R-values:
R R work R free
0.226 0.224 0.275
Expression system: Trichoplusia ni

Quick links

Citations

15 review citations

Role of receptor binding specificity in influenza A virus transmission and pathogenesis.
de Graaf et al. (2014)
14 more

10 mentions without citation

A recommended numbering scheme for influenza A HA subtypes.
Burke et al. (2014)
9 more