› 4k14

X-ray diffraction
1.6Å resolution

Crystal Structure of Staphylococcal nuclease mutant V66I/V99L

Released:
DOI: 10.2210/pdb4k14/pdb
PDB entry 4k14 coloured by chain, front view.
PDB entry 4k14 coloured by chain, side view.
PDB entry 4k14 coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry authors: Janowska K, Sakon J, Stites WE

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.57 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00644 (Residues: 88-223; Coverage: 66%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P41
Unit cell:
a: 47.45Å b: 47.45Å c: 63.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.252
Expression system: Escherichia coli

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