› 4ky9

X-ray diffraction
2.23Å resolution

Structural and Functional Analysis of a Putative Substrate Access Tunnel in the Cytosolic Domain of Human Anion Exchanger 1

Released:
DOI: 10.2210/pdb4ky9/pdb
PDB entry 4ky9 coloured by chain, front view.
PDB entry 4ky9 coloured by chain, side view.
PDB entry 4ky9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A substrate access tunnel in the cytosolic domain is not an essential feature of the solute carrier 4 (SLC4) family of bicarbonate transporters.
Shnitsar V, Li J, Li X, Calmettes C, Basu A, Casey JR, Moraes TF, Reithmeier RAF
J Biol Chem 288 33848-33860 (2013)
PMID: 24121512

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136111 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Band 3 anion transport protein Chains: A, P
Molecule details ›
Chains: A, P
Length: 306 amino acids
Theoretical weight: 34.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02730 (Residues: 51-356; Coverage: 34%)
Gene names: AE1, DI, EPB3, SLC4A1
Sequence domains: Band 3 cytoplasmic domain
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 69.905Å b: 80.7Å c: 104.278Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Bicarbonate transport in health and disease.
Alka et al. (2014)
4 more

11 mentions without citation

Organization and Dynamics of the Red Blood Cell Band 3 Anion Exchanger SLC4A1: Insights From Molecular Dynamics Simulations.
Kalli et al. (2022)
10 more