› 4mnt

X-ray diffraction
1.58Å resolution

Crystal structure of human DJ-1 in complex with Cu

Released:
DOI: 10.2210/pdb4mnt/pdb
PDB entry 4mnt coloured by chain, front view.
PDB entry 4mnt coloured by chain, side view.
PDB entry 4mnt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DJ-1 is a copper chaperone acting on SOD1 activation.
Girotto S, Cendron L, Bisaglia M, Tessari I, Mammi S, Zanotti G, Bubacco L
J Biol Chem 289 10887-10899 (2014)
PMID: 24567322

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 19.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3121
Unit cell:
a: 75.24Å b: 75.24Å c: 75.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.151 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Oxidative stress: A major pathogenesis and potential therapeutic target of antioxidative agents in Parkinson's disease and Alzheimer's disease.
Jiang et al. (2016)
22 more

1 mention without citation

DJ-1 is a copper chaperone acting on SOD1 activation.
Girotto et al. (2014)