› 4moe

X-ray diffraction
2Å resolution

Pyranose 2-oxidase H450G mutant with 3-fluorinated glucose

Released:
DOI: 10.2210/pdb4moe/pdb
PDB entry 4moe coloured by chain, front view.
PDB entry 4moe coloured by chain, side view.
PDB entry 4moe coloured by chain, top view.
Source organism: Trametes ochracea
Primary publication:
Structural basis for binding of fluorinated glucose and galactose to Trametes multicolor pyranose 2-oxidase variants with improved galactose conversion.
Tan TC, Spadiut O, Gandini R, Haltrich D, Divne C
OpenAccess logo PLoS One 9 e86736 (2014)
PMID: 24466218

Function and Biology Details

Reaction catalysed: 
D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyranose 2-oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 633 amino acids
Theoretical weight: 70.47 KDa
Source organism: Trametes ochracea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7ZA32 (Residues: 1-622; Coverage: 100%)
Gene name: p2o
Sequence domains: GMC oxidoreductase
Structure domains: Phage tail proteins - 2 layer sandwich fold

Ligands and Environments


Cofactor: Ligand FDA 4 x FDA
3 bound ligands:
Ligand G3F 4 x G3F
Ligand 12P 4 x 12P
Ligand MES 5 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21
Unit cell:
a: 100.068Å b: 102.742Å c: 137.692Å
α: 90° β: 90.8° γ: 90°
R-values:
R R work R free
0.196 0.195 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Experimental and Computational Studies on Structure and Energetic Properties of Halogen Derivatives of 2-Deoxy-D-Glucose.
Ziemniak et al. (2021)
2 more

4 mentions without citation

Metal based donepezil analogues designed to inhibit human acetylcholinesterase for Alzheimer's disease.
Junaid et al. (2019)
3 more