› 4ndz

X-ray diffraction
3.45Å resolution

Structure of Maltose Binding Protein fusion to 2-O-Sulfotransferase with bound heptasaccharide and PAP

Released:
DOI: 10.2210/pdb4ndz/pdb
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PDB entry 4ndz coloured by chain, front view.
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PDB entry 4ndz coloured by chain, top view.
Source organisms:
Entry authors: Liu C, Sheng J, Krahn JM, Perera L, Xu Y, Hsieh P, Liu J, Pedersen LC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Heparan sulfate 2-O-sulfotransferase 1; Maltose/maltodextrin-binding periplasmic protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 658 amino acids
Theoretical weight: 74.4 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-393; Coverage: 99%)
  • Canonical: Q76KB1 (Residues: 69-356; Coverage: 81%)
Gene names: HS2ST, HS2ST1, JW3994, b4034, malE
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

Carbohydrate polymer : NEW Components: BDP, GNS, IDR, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand A3P 6 x A3P
Ligand NPO 4 x NPO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 155.72Å b: 170.69Å c: 183.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.228
Expression system: Escherichia coli

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