› 4nuf

X-ray diffraction
2.8Å resolution

Crystal Structure of SHP/EID1

Released:
DOI: 10.2210/pdb4nuf/pdb
PDB entry 4nuf coloured by chain, front view.
PDB entry 4nuf coloured by chain, side view.
PDB entry 4nuf coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into gene repression by the orphan nuclear receptor SHP.
Zhi X, Zhou XE, He Y, Zechner C, Suino-Powell KM, Kliewer SA, Melcher K, Mangelsdorf DJ, Xu HE
Proc Natl Acad Sci U S A 111 839-44 (2014)
PMID: 24379397

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142575 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin-binding periplasmic protein; Nuclear receptor subfamily 0 group B member 2 Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 64.19 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
  • Canonical: Q62227 (Residues: 55-260; Coverage: 79%)
Gene names: JW3994, Nr0b2, Shp, b4034, malE
Sequence domains:
Structure domains: Retinoid X Receptor
EP300-interacting inhibitor of differentiation 1 Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 1.82 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9DCR4 (Residues: 91-106; Coverage: 10%)
Gene names: Cri1, Eid1

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 56.388Å b: 105.148Å c: 136.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

The nuclear receptor superfamily: A structural perspective.
Weikum et al. (2018)
3 more

3 mentions without citation

Crystal structures of MBP fusion proteins.
Waugh DS. (2016)
2 more