› 4o6q

X-ray diffraction
0.95Å resolution

0.95A resolution structure of the hemophore HasA from Pseudomonas aeruginosa (Y75A mutant)

Released:
DOI: 10.2210/pdb4o6q/pdb
PDB entry 4o6q coloured by chain, front view.
PDB entry 4o6q coloured by chain, side view.
PDB entry 4o6q coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Replacing the axial ligand tyrosine 75 or its hydrogen bond partner histidine 83 minimally affects hemin acquisition by the hemophore HasAp from Pseudomonas aeruginosa.
Kumar R, Matsumura H, Lovell S, Yao H, Rodríguez JC, Battaile KP, Moënne-Loccoz P, Rivera M
OpenAccess logo Biochemistry 53 2112-25 (2014)
PMID: 24625274

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme acquisition protein HasA Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 18.81 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: O69756 (Residues: 1-184; Coverage: 90%)
Gene names: IPC737_16450, hasAp
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

2 bound ligands:
Ligand FMT 1 x FMT
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 34.437Å b: 46.729Å c: 101.037Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.135 0.146
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Extracellular Heme Uptake and the Challenge of Bacterial Cell Membranes.
Huang et al. (2017)
1 more

2 mentions without citation

Axial Heme Coordination by the Tyr-His Motif in the Extracellular Hemophore HasAp Is Critical for the Release of Heme to the HasR Receptor of Pseudomonas aeruginosa.
Dent et al. (2021)
1 more